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Protocol 2: Intermediate approach (experienced researchers) Goal: Multi-target immune modulation with anti-inflammatory support Components: Thymosin alpha-1: 1.6 mg subcutaneously, three times weekly KPV: 200-500 mcg subcutaneously, once daily BPC-157: 250 mcg subcutaneously, twice daily Selank: 250-500 mcg intranasally, once daily Schedule: Monday, Wednesday, Friday: Thymosin alpha-1 Daily AM: KPV + BPC-157 Daily PM: BPC-157 Daily: Selank nasal spray (morning or midday) Duration: 12 weeks on, 4 weeks off Monitoring: Baseline labs, repeat every 4 weeks

doi: 10.1016/j.ijbiomac.2021.08.169 9 ChaudhuriA.HalderK.DattaA

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Studies show that TLR4 and COX-2 levels are elevated in prostate cancer, and reducing their expression can inhibit cell proliferation, migration, and invasion by lowering p65 phosphorylation

Endothelial function and vascular oxidative stress in long-lived GH/IGF-deficient Ames dwarf mice